Methodology

High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi

Peter Hohenstein, Joan Slight, Derya D Ozdemir, Sally F Burn, Rachel Berry and Nicholas D Hastie^*

MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK

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PathoGenetics 2008, 1:3 doi:10.1186/1755-8417-1-3

Published: 3 November 2008

Additional file 1:

VectorNTI archive file for pRoas26-DEST.

Format: MA4 Size: 16KB Download file

Additional file 2:

pRosa26-DEST sequence in genbank format.

Format: TXT Size: 21KB Download file

Additional file 3:

pRosa26-DEST map.

Format: PDF Size: 65KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 4:

pRosa26-DEST information file including protocols.

Format: PDF Size: 140KB Download file

This file can be viewed with: Adobe Acrobat Reader